The high street fashion retailer told staff it intends to slash store management and senior sales roles as part of a restructure of its retail team.
The move comes just a month after it had already said it would cut 250 head office staff as part of cost-cutting measures.
River Island has seen revenues and profitability hit by a slump in store footfall after reopening sites following the coronavirus lockdown.
Chief executive Will Kernan told staff in an internal memo that the changes will create a “flatter management structure with a greater emphasis on customer service”.
He said: “We need to make sure we have the right structures in place to deliver our omnichannel strategy, and to continue to deliver the amazing River Island in-store experience that our customers know and love.